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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
20/01/2011 |
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Data da última atualização: |
07/07/2016 |
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Autoria: |
CÂNDIDO, M. J. D.; BEZERRA, A. P. A.; VOLTOLINI, T. V.; BELÉM, K. V. J.; SOUZA, R. A.; MORAES, S. A. de. |
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Afiliação: |
MAGNO JOSÉ DUARTE CÂNDIDO; ANA PATRÍCIA ALMEIDA BEZERRA; TADEU VINHAS VOLTOLINI, CPATSA; KAIO VICTOR JUSTO BELÉM; RAFAEL ARAÚJO SOUZA; SALETE ALVES DE MORAES, CPATSA. |
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Título: |
Peso e rendimento de carcaça de borregos pastejando capim tifton 85 sob três períodos de descanso e dois resíduos pós-pastejo. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 47., 2010, Salvador. Empreendedorismo e progresso científico na zootecnia brasileira: anais. Salvador: SBZ, 2010. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Avaliação; Capim Tifton 85; Cynodon sp; Rendimento biológico; Rendimento comercial; Rendimento verdadeiro. |
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Thesagro: |
Carcaça; Ovino; Pastagem. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00974naa a2200289 a 4500
001 1873703
005 2016-07-07
008 2010 bl uuuu u00u1 u #d
100 1 $aCÂNDIDO, M. J. D.
245 $aPeso e rendimento de carcaça de borregos pastejando capim tifton 85 sob três períodos de descanso e dois resíduos pós-pastejo.
260 $c2010
300 $c1 CD-ROM.
650 $aCarcaça
650 $aOvino
650 $aPastagem
653 $aAvaliação
653 $aCapim Tifton 85
653 $aCynodon sp
653 $aRendimento biológico
653 $aRendimento comercial
653 $aRendimento verdadeiro
700 1 $aBEZERRA, A. P. A.
700 1 $aVOLTOLINI, T. V.
700 1 $aBELÉM, K. V. J.
700 1 $aSOUZA, R. A.
700 1 $aMORAES, S. A. de
773 $tIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 47., 2010, Salvador. Empreendedorismo e progresso científico na zootecnia brasileira: anais. Salvador: SBZ, 2010.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca |
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Classificação |
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Registro |
Volume |
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URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
23/11/2017 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
PINTO, I. S. B.; FONSECA, I.; BRANDAO, H. de M.; GERN, J. C.; GUIMARAES, A. S.; CARVALHO, W. A.; BRITO, M. A. V. P. e; VICCINI, L. F.; MARTINS, M. F. |
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Afiliação: |
ISABELLA S. B. PINTO, CNPq; ISABELA FONSECA, CNPq; HUMBERTO DE MELLO BRANDAO, CNPGL; JULIANA CARINE GERN, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; WANESSA ARAUJO CARVALHO, CNPGL; MARIA APARECIDA V PAIVA E BRITO, CNPGL; LYDERSON FACIO VICCINI, UFJF; MARTA FONSECA MARTINS, CNPGL. |
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Título: |
SHORT-COMMUNICATION: Evaluation of perfused bovine udder for gene expression studies in dairy cows. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Genetic and Molecular Research, v. 16, n. 1, 2017. |
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Páginas: |
7 p. |
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DOI: |
10.4238/gmr16019637 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Intramammary infections are one of the main causes of productivity loss in dairy cows. To better understand the immune system response and to avoid the use of live animals, we validated the use of isolated bovine udder as an ex situ model. Six mammary glands were collected from cows ready for culling. Three udders were perfused with Tyrode's solution and three were not-perfused. During six hours, we collected perfusate samples for biochemical analysis. We also collected alveolar and teat canal tissue to evaluate gene expression. The biochemical parameters indicated that the perfused udders remained viable for the entire period of the experiment. A real-time polymerase chain reaction showed an increase in 18S rRNA gene expression in the alveolar tissue at 3 and 4 h after perfusion. There was also an increase in the Ubiquitin gene in the teat canal from not-perfused udders at 1, 3, and 4 h after slaughter. In general, gene expression was stable during the experiment. Our results indicated that the isolated perfused bovine udder model is appropriate for genetic studies, opening a new perspective in animal experimentation methods. |
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Palavras-Chave: |
Ex-situ model; Mammary gland. |
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Thesaurus NAL: |
genes. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/167362/1/Cnpgl-2017-ShortComm-GMR-Evaluation.pdf
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Marc: |
LEADER 01949naa a2200277 a 4500
001 2080581
005 2023-01-27
008 2017 bl uuuu u00u1 u #d
024 7 $a10.4238/gmr16019637$2DOI
100 1 $aPINTO, I. S. B.
245 $aSHORT-COMMUNICATION$bEvaluation of perfused bovine udder for gene expression studies in dairy cows.$h[electronic resource]
260 $c2017
300 $a7 p.
520 $aAbstract Intramammary infections are one of the main causes of productivity loss in dairy cows. To better understand the immune system response and to avoid the use of live animals, we validated the use of isolated bovine udder as an ex situ model. Six mammary glands were collected from cows ready for culling. Three udders were perfused with Tyrode's solution and three were not-perfused. During six hours, we collected perfusate samples for biochemical analysis. We also collected alveolar and teat canal tissue to evaluate gene expression. The biochemical parameters indicated that the perfused udders remained viable for the entire period of the experiment. A real-time polymerase chain reaction showed an increase in 18S rRNA gene expression in the alveolar tissue at 3 and 4 h after perfusion. There was also an increase in the Ubiquitin gene in the teat canal from not-perfused udders at 1, 3, and 4 h after slaughter. In general, gene expression was stable during the experiment. Our results indicated that the isolated perfused bovine udder model is appropriate for genetic studies, opening a new perspective in animal experimentation methods.
650 $agenes
653 $aEx-situ model
653 $aMammary gland
700 1 $aFONSECA, I.
700 1 $aBRANDAO, H. de M.
700 1 $aGERN, J. C.
700 1 $aGUIMARAES, A. S.
700 1 $aCARVALHO, W. A.
700 1 $aBRITO, M. A. V. P. e
700 1 $aVICCINI, L. F.
700 1 $aMARTINS, M. F.
773 $tGenetic and Molecular Research$gv. 16, n. 1, 2017.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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